Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p327 | (1) | WCRB2014

Establishment of immortalized neuronal cell lines derived from fetal goat hypothalamus

Suetomi Yuta , Tsukamura Hiroko , Ohkura Satoshi , Matsuda Fuko

Neurons essential for reproductive functions are distributed in hypothalamus. The precise functions of these neurons are not fully analyzed at a cellular level, especially in domestic animals. To analyze cellular mechanisms that control ruminant reproduction, we generated neuronal cell lines by immortalizing fetal goat hypothalamus.The hypothalamic arcuate nucleus was collected from a female Shiba goat at E118. The tissue was dispersed and plated. Twenty...